ABSTRACT
OBJECTIVE To investigate the improve-ment functions of flavonoid compounds on temozolomide(TMZ)-,aging-or AD model-induced dysregulation of hip-pocampal NSC lineage progression,retardancy of den-dritic spine maturation in new-born neurons,as well as impairment of hippocampal-related learning and memory.METHODS We applied 30-week-old neural stem cell(NSC)specific promoter Nestin-GFP and NestinCreERT2:Rosa26-LSL-tdTomato transgenic mice and 16-week-old AD model 5XFAD transgenic mice,together with hippo-campal microinjection(ih),endogenous fluorescence trac-ing and immunofluorescent staining.RESULTS Both fla-vonoid compound A and its functional derivative flavo-noid compound B dose-dependently improved TMZ-,aging-or AD-induced defects of hippocampal NSC lin-eage progression and the maturation of dendritic spines of newborn neurons,thereby improving hippocampus related learning and memory.CONCLUSION This paper provides a new idea and treatment strategy for the devel-opment of new flavonoids that can promote neurogene-sis for neurodegenerative diseases and aging.
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β-Thalassemia is a global health issue, caused by mutations in the HBB gene. Among these mutations, HBB -28 (A>G) mutations is one of the three most common mutations in China and Southeast Asia patients with β-thalassemia. Correcting this mutation in human embryos may prevent the disease being passed onto future generations and cure anemia. Here we report the first study using base editor (BE) system to correct disease mutant in human embryos. Firstly, we produced a 293T cell line with an exogenous HBB -28 (A>G) mutant fragment for gRNAs and targeting efficiency evaluation. Then we collected primary skin fibroblast cells from a β-thalassemia patient with HBB -28 (A>G) homozygous mutation. Data showed that base editor could precisely correct HBB -28 (A>G) mutation in the patient's primary cells. To model homozygous mutation disease embryos, we constructed nuclear transfer embryos by fusing the lymphocyte or skin fibroblast cells with enucleated in vitro matured (IVM) oocytes. Notably, the gene correction efficiency was over 23.0% in these embryos by base editor. Although these embryos were still mosaic, the percentage of repaired blastomeres was over 20.0%. In addition, we found that base editor variants, with narrowed deamination window, could promote G-to-A conversion at HBB -28 site precisely in human embryos. Collectively, this study demonstrated the feasibility of curing genetic disease in human somatic cells and embryos by base editor system.
Subject(s)
Female , Humans , APOBEC-1 Deaminase , Genetics , Metabolism , Base Sequence , Blastomeres , Cell Biology , Metabolism , CRISPR-Cas Systems , Embryo, Mammalian , Metabolism , Pathology , Fibroblasts , Metabolism , Pathology , Gene Editing , Methods , Gene Expression , HEK293 Cells , Heterozygote , Homozygote , Point Mutation , Primary Cell Culture , Promoter Regions, Genetic , Sequence Analysis, DNA , beta-Globins , Genetics , Metabolism , beta-Thalassemia , Genetics , Metabolism , Pathology , TherapeuticsABSTRACT
To evaluate the gB antibody level of pseudorabies virus (PRV) in swine,a rapid test strip was developed.In the strip,the expressed protein of gB was labeled with colloidal gold,the staphylococcal protein A (SPA) and swine anti PRV antibody were blotted on the nitrocellulose membrane for the test and control lines,respectively.The specificity and sensitivity of the strip were detected with standard positive,negative and immunized sera of PRV,the results indicated that the strip was high specificity and sensitivity.Field swine serum samples were tested by the new strip and commercial IDEXX PRV gB ELISA kit,simultaneously.The agreement rate of the two methods was 91.04％.Furthermore,the dipstick assay based on the strip is rapid (5 min),sensitive and easy to perform.This suggests that the new strip is an acceptable alternative for field diagnosis.
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<p><b>OBJECTIVE</b>To study the Arbuscular mycorrhizal (AM) formation progress and infection characteristics between tissue culture plantlets of Pinellia ternata and Glomus mosseae.</p><p><b>METHOD</b>The tissue culture plantlets of P. ternata were inoculated with G. mosseae, the formation of AM were sampled and observed with microscopy by staining.</p><p><b>RESULT AND CONCLUSION</b>The hyphae of G. mosseae began to penetrate the root epidermis after 10 days of inoculation. Lots of intracellular hyphae formed in cortex cells at the 15th day. Arbuscules started to form and there were some hyphae on the root at the 20th day. At the 25th day, many arbuscules formed and most as Arum type. Some arbuscles started to disintegrate at the 30th day, and a few of vesicles occurred. Lots of spores formed after 35 days. At the 40th day, some vesicles began to decline. The hand section showed that the intercellular hyphae gradually formed in intercellular space, and the hyphae branched in cortex cells and occupied most cell lumen finally. It is expounded that P. ternata and G. mosseae could recognize each other quickly and form a symbiont system.</p>
Subject(s)
Cell Culture Techniques , Cells, Cultured , Glomeromycota , Physiology , Hyphae , Physiology , Mycorrhizae , Physiology , Pinellia , Cell Biology , MicrobiologyABSTRACT
Art phonetics' medicine, a new branch of traditional medicine, has not been developed perfectly, especially in the aspects of objective and scientific study. In this paper, the acoustical and anatiomical basis of art phonetics in viewpoint of biomedical engineering is explored, and then our work of quantitative measurement and analysis of art phonetic is introduced. The experiment data show further that quantitative measurement and analysis plays an important role in art phonetic medicine.
Subject(s)
Female , Humans , Male , Acoustics , Biomedical Engineering , Image Processing, Computer-Assisted , Larynx , PhoneticsABSTRACT
Objective To obtain the normal ranges of different parameters in static posturography.Methods Different parameters' values of 117 normal persons (10～19 years old) were detected by static posturography while they were in opening and closing eyes situations. Results The mean values of different parameters in opening eyes situation are significantly lower that in closing eyes situation(P＜0.01). The values from the male and the female demonstrate no significant difference, values from 10～19 years old group and 70～79 years old group are significantly higher than from 20～69 years old group(P＜0.01). Conclusion The static posturography is a perfect measure to analyze and evaluate the balance ability of human body in exact quantification, and could be one of the routine clinic tests, supplying quantificational, intellective and facile method for diagnosis and evaluation of dizziness and balance ability.
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Objective To find out the changes of voice in different noisy background by computer acoustic analysis.Methods 25 young people with normal hearing and normal vocal cords were selected.Sustained /a/ and /i/ were recorded in three different background:quiet, white band noise and speech noise. The software of Multi Speech and Doctor Speech were used. Testing parameters included: Fo, mean amplitudes, standard deviations of amplitudes,F 1, F 2.Results Fundamental frequencies of noisy groups were significantly higher in male-/a/, female-/a/ and female-/i/.F 1 of noisy groups were significantly higher in male-/a/, male-/i/ and female-/i/.Conclusion Fo and F 1 of normal people can be changed by noise of backgroud.
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RNA dot blot hybridization was employed to investigate the alterations in C-myc, C-H-ras transcript levels in bone marrow and peripheral blood cells from 24 patients with acute nonlymphocytic leukemia. 10 cases of normal bone marrow cells were used as control. We found that the positive rates and intensities of expression of C-myc and C-H-ras in acute nonlymphocytic leukemia were hipher than those of normal control. The C-myc and C-H-ras expressed simultaneously in 10 patients. After having been cultured 5 days with RA and low dose Ara-C combined with low dose Harr, the C-myc transcipt level of C-myc in acute nonlymphocytic leukemia was decreased obviously.